human normal liver cell line thle3 (ATCC)
Structured Review

Human Normal Liver Cell Line Thle3, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 256 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+normal+liver+cell+line+thle3/pmc07812052-74-0-11?v=ATCC
Average 96 stars, based on 256 article reviews
Images
1) Product Images from "microRNA-19a-3p and microRNA-376c-3p Promote Hepatocellular Carcinoma Progression Through SOX6-Mediated Wnt/β-Catenin Signaling Pathway"
Article Title: microRNA-19a-3p and microRNA-376c-3p Promote Hepatocellular Carcinoma Progression Through SOX6-Mediated Wnt/β-Catenin Signaling Pathway
Journal: International Journal of General Medicine
doi: 10.2147/IJGM.S278538
Figure Legend Snippet: miR-19a-3p and miR-376c-3p are highly expressed in HCC tissues and cells. ( A ) Microarray-based analysis of differentially expressed miRNAs between HCC tissues and adjacent normal tissues. ( B ) miR-19a-3p and miR-376c-3p expression in 21 cases of HCC tissues and adjacent normal tissues tested by RT-qPCR. ( C ) miR-19a-3p and miR-376c-3p expression in human normal liver cell line THLE3 and HCC cell lines (Hep3B, MHCC-97L, Huh7) determined by RT-qPCR. ( D ) detection of miR-19a-3p expression in the TCGA database. ( E ) detection of miR-19a-3p expression at different stages of HCC. ( F ) analysis of the prognosis of patients with differential expression of miR-19a-3p. ( G ) detection of miR-376c-3p expression in the TCGA database. ( H ) detection of miR-376c-3p expression at different stages of HCC. ( I ) analysis of the prognosis of patients with differential expression of miR-376c-3p. The measurement data were depicted as mean ± standard deviation. The data between two groups were compared by unpaired t -test or and the data among multiple groups were compared by two-way ANOVA. The experiment was repeated three times. * p < 0.05 vs adjacent normal tissues or THLE3 cells.
Techniques Used: Microarray, Expressing, Quantitative RT-PCR, Quantitative Proteomics, Standard Deviation
Figure Legend Snippet: SOX6 is poorly expressed in HCC tissues and cells. ( A ) SOX6 mRNA expression in 21 cases of HCC tissues and adjacent normal tissues determined by RT-qPCR. ( B ) The positive rate of SOX6 protein expression in 21 cases of HCC tissues and adjacent normal tissues detected by immunohistochemical staining. ( C ) SOX6 mRNA expression in human normal liver cell line THLE3 and HCC cell lines (Hep3B, MHCC-97L, Huh7) determined by RT-qPCR. ( D ) SOX6 protein expression in human normal liver cell line THLE3 and HCC cell lines (Hep3B, MHCC-97L, Huh7) tested by Western blot analysis. ( E ) detection of SOX6 expression in the TCGA database. ( F ) detection of SOX6 expression at different stages of HCC. ( G ) analysis of the prognosis of patients with differential expression of SOX6. ( H ) correlation analysis of miR-19a-3p and miR-376c-3p with SOX6 expression in HCC tissues. The measurement data were depicted as mean ± standard deviation. Comparison between two groups was conducted by unpaired t -test or and comparisons among multiple groups were analyzed by one-way ANOVA. The experiment was repeated three times. * p < 0.05 vs adjacent normal tissues or THLE3 cells.
Techniques Used: Expressing, Quantitative RT-PCR, Immunohistochemical staining, Staining, Western Blot, Quantitative Proteomics, Standard Deviation, Comparison